"Output Type", the area below the lower threshold is considered a background. The upper threshold of the image is ignored. "Above Lower Threshold" - (This option appears for thresholded images only.)įinds maxima above the lower threshold only.Check Light Background if the image background is brighter than the objects you want to find,Īs it is in the Cell Colony image in the illustration above.The edge of the image (edge of the selection does not matter). "Exclude Edge Maxima" - Excludes maxima if the area within the noise tolerance surrounding a maximum touches."Count" - Displays the number of maxima in the Results window."Display Point selection" - Displays a multi-point selection with a point at each maximum. Process>Binary>Watershed, which uses the Euclidian distance map). The image by a watershed algorithm applied to the values of the image (in contrast to "Segmented Particles" - Assumes that each maximum belongs to a particle and segments."Maxima Within Tolerance" - all points within the "Noise Tolerance" for each maximum."Single Points" - results in one single point per maximum.Only one maximum within this area is accepted. For accepting a maximum, this area must not containĪny point with a value higher at than the maximum. In other words, a threshold is set at the maximum value minus noise tolerance and theĬontiguous area around the maximum above the threshold is analyzed. (calibrated units for calibrated images). "Noise Tolerance" - Maxima are ignored if they do not stand out from the surroundings by more than this value.ThisĬommand is based on a plugin contributed by Michael Schmid.Ī dialog box is displayed with the following options: Unweighted average of the colors depending on the Edit>Options>Conversions settings. With the maxima, or one segmented particle per maximum, marked.įor RGB images, maxima of luminance are selected, with the luminance defined as weighted or The final image is produced by combining the two derivatives using the square root of the sum of the squares.ĭetermines the local maxima in an image and creates a binary (mask-like) image of the same size Two 3x3 convolution kernels (show below) are used to generate vertical and horizontal derivatives. Uses a Sobel edge detector to highlight sharp changes in intensity in the active image or selection. This filter uses the following weighting factors to replace each pixel with a weighted average of the 3x3 neighborhood. Increases contrast and accentuates detail in the image or selection, but may also accentuate noise. This filter replaces each pixel with the average of its 3x3 neighborhood. Home | contents | previous | next Process Menuīlurs the active image or selection.
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